Step
01
- Sampling and written consent
Sample Preparation
01 Specimen Collection Container
1) DNase-free microcentrifuge tube or cryovial
02 Sampling Method
1) Extract the DNA using a commercialized kit or via SDS/Proteinase K digestion, phenol-chloroform extraction, or EtOH ppt method
2) CYP2D6/2A6 must be dissolved in sterilized distilled water
03 Storage Temperature and Period
1) Refrigerated (4℃): to be received within a week
2) Frozen (≤-20 ℃): to be received within 12 months
3) Deep freeze (≤-80 ℃): to be received within 48 months
4) Freezing and melting should be performed no more than 10 times (no more than 5 times for CYP2D6/2A6)
04 Sample Amount
1) At least 1ug (20ng/ul)
2) May increase/decrease the required amount depending on gene and mutation characteristics
05 Precautions
1) 260nmOD/280nmDO ratio: 1.6≤R≤2.0
2) The DNA should be double stranded and not be degraded
3) Bands in appropriate sizes should be identified during electrophoresis
4) In the case of DNA molecules that are over 5kb in size (CYP2D6/CYP2A6, etc.), there should be no PCR inhibitor.
5) Do not apply heat treatment or any method that involves the use of a strong denaturant, as this may cause the DNA to become single stranded during isolation.
1) DNase-free microcentrifuge tube or cryovial
02 Sampling Method
1) Extract the DNA using a commercialized kit or via SDS/Proteinase K digestion, phenol-chloroform extraction, or EtOH ppt method
2) CYP2D6/2A6 must be dissolved in sterilized distilled water
03 Storage Temperature and Period
1) Refrigerated (4℃): to be received within a week
2) Frozen (≤-20 ℃): to be received within 12 months
3) Deep freeze (≤-80 ℃): to be received within 48 months
4) Freezing and melting should be performed no more than 10 times (no more than 5 times for CYP2D6/2A6)
04 Sample Amount
1) At least 1ug (20ng/ul)
2) May increase/decrease the required amount depending on gene and mutation characteristics
05 Precautions
1) 260nmOD/280nmDO ratio: 1.6≤R≤2.0
2) The DNA should be double stranded and not be degraded
3) Bands in appropriate sizes should be identified during electrophoresis
4) In the case of DNA molecules that are over 5kb in size (CYP2D6/CYP2A6, etc.), there should be no PCR inhibitor.
5) Do not apply heat treatment or any method that involves the use of a strong denaturant, as this may cause the DNA to become single stranded during isolation.
Required documents
01Written consent for genetic testing
02Genetic testing application
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